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Agarose
Product Introduction:
Agarose is a purified, linear galactan hydrophilic colloid extracted from agar or algae containing agar. Structurally, it is a linear polymer composed of β-D-pyranogalactose units linked by (1→4) bonds, interspersed with 3,6-dehydrated α-L-pyranogalactose residues. As a gel-forming agent, agarose is commonly used in routine nucleic acid analysis via gel electrophoresis or blotting techniques (such as Northern or Southern blotting). It is also suitable for protein applications, including radial immunodiffusion (RID) assays.
Basic parameters of agarose, including:
1) Sulfate content—A purity indicator, as the sulfate group is the primary ionic moiety present in agarose;
2) Gel strength—The external force applied to a gel that causes it to break;
3) Gel point—The temperature at which a water-soluble agarose solution forms a gel upon cooling. During the transition from liquid to gel, agarose solutions exhibit hysteresis; therefore, the gel point is not identical to the gel melting point.
4) Electroosmotic Flow (EEO)—an electrokinetic phenomenon in which liquid moves through a gel. In agarose gels, the anionic groups adsorbed onto the matrix do not migrate; however, dissociated cations will migrate toward the negative electrode, thereby generating electroosmotic flow. Since the electrophoretic migration of biological polymers typically occurs toward the negative electrode, the internal convection induced by EEO can interfere with separation efficiency.
Scope of application:
— Gel electrophoresis
— Imprint
—Ouchterlony immunodiffusion
— Radial Immunodiffusion
— Background dyes such as SYBR Green
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Technical Data Sheet
| Appearance | White to off-white powder |
| Gel strength (g/cm²) | ≥1200 |
| Gelation temperature range | 36 ± 1.5°C |
| Melting temperature range | 88 ± 1.5°C |
| Electroosmotic value | ≤0.13 |
| Sulfate | ≤0.15% |
| Moisture | ≤10% |
| DNA enzyme | Not detectable |
| RNAase | Not detectable |
| Protease | Not detectable |
